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Population structure was inferred using the STRUCTURE program [ 45], which seeks solutions for a given number of clusters K applied to genotypic data in such a way that each cluster is in or close to Hardy-Weinberg and linkage equilibrium [ 46].
(XLSX 30 kb) Additional file 7: The ∆K value of the population genetic structure of the 26 Brassica accessions generated using the STRUCTURE program calculation with different data set of markers.
In TUK, significant population substructure was excluded using the STRUCTURE program.
To reduce the Wahlund effect, the samples collected in each of the growing areas were divided according to the genetic clusters evidenced using the Structure program.
The most appropriate number was obtained by testing K values from 1 to 15 using the Structure program (version 2.2) [ 40].
The two clusters inferred using the Structure program without making use of sample group information to assist clustering were explained neither by the type of symptom nor by the geographic origin of isolates.
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To gain further structural insight, we generated a structure-based phylogenetic tree for these homologous helicases (Fig. 1D), using the Structure Homology Program (Stuart et al., 1979).
The most likely number of population clusters was estimated by the ∆K parameter [ 76] using the Structure Harvester online program [ 77].
The structures were solved by the molecular replacement method with the Phaser program using the structure of the human H3.3 nucleosome (PDB ID: 3AV2) as the search model64,67.
To generate models for the substrate lysine and a functionally important N-lobe loop not visible in the Rsp5WW3-HECTxUbxSna3C electron density, we used the structure prediction program Rosetta.
We analysed the MLGs that were assigned to cluster B and those that were assigned to cluster A separately using the same parameters of the Structure program.
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