Sentence examples for using the strategy described from inspiring English sources

To further confirm that the PTPase activity of SHP2 was responsible for the observed morphological changes and to rule out the possibility of any off-target shRNA effects, we inhibited SHP2 function by expression of the PTPase-dead SHP2 C459S-SHP22), also known as dominant-negative SHP2, using the strategy described by us previously [ 12, 13].

We next generate a point mutation strain Mosdi1 R using the strategy described in Fig. 2b, and found that, like many other fungi, amino acid substitution (H245L) in the Mosdi1 subunit (Mosdi1 R mutants) could confer resistance to carboxin (Fig. 2a).

Mutants of M. bovis BCG and M. tuberculosis were constructed using the strategy described by Stewart et al, 2001.

We searched in the DNA region immediately upstream of each 3' cDNA end for promoter elements recognized by E-σ70 and E-σ38 using the strategy described above.

Spoligotyping and MIRU-VNTR patterns were submitted to MIRU-VNTRplus (www.miru-vntrplus.org) [38] for lineage identification, using the strategy described in [32], [38].

For knock down of fipAMTB, an antisense construct (pJB119) of fipA was generated by PCR amplification of the fipA gene using the primer pair FipAMTB sense and FipAMTB antisense (Table S2) followed by cloning of the PCR product in reverse orientation under the control of the hsp60 promoter using the strategy described by Sureka et al. [48].

In Study 3, time spent using the strategies described in Study 2 was controlled.

Using the strategies described in this study, the protective effect of compounds on muscle degeneration could be conducted rapidly.

These concerns were addressed using the strategies described above.

Using the strategies described here would address the concerns raised by young women in this study, and help to ensure the greatest population coverage of chlamydia screening.

However, the frequency of valid SNPs for linkage analysis was low because of several difficulties in developing informative markers using the strategies described in this paper.