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All statistical evaluation of data was done by using the statistic program SPSS Statistics v. 19.
Data were processed using the statistic program SPSS1201 version 7.0.1.4.
Data were analyzed using the statistic program StatView® 5.0 (SAS Institute Inc., Cary, NC, USA).
Using the statistic program Graph Pad Prism 4 and the Student's t test the ratios of different samples were tested for significance.
To determine statistical significance, quantitative analyses of the LVA, LVN and LVD in all analysed areas (intratumoural, 50 μm, 50 200 μm, 200 300 μm and >300 μm peritumoural) were performed in a standardised procedure using the statistic program InStat 3 (GraphPad Software, San Diego, California).
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Statistical evaluation of the data obtained was performed using the statistic software program SPSS Version 20 (IBM Corporation, Armonk, NY, US).
Data analysis was performed using the statistic-analyzing program JMP® (Version 5.0.1.2, SAS Institute, Cary, NC, USA).
The extent of LD was determined using the D' statistic.
The selected allometric equations were tested for statistical significance using the R Statistic Program (Additional file 1: 1).
Analysis was performed using the SPSS statistic program (version 10.0 for Windows) (SPSS Inc, Chicago, IL, USA).
All data were analysed using the PASW statistic program (SPSS 20.0, Chicago, IL, USA).
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