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Sequence type (ST) and alleles were analyzed by UPGMA (unweighted pair group method with arithmetic mean) and BURST (based upon related STs) analysis by using the START program (version 1.0.5 [ 28]) to assign lineage and clonal complexes.
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The data were used to draw an unweighted pair group mean average (UPGMA) dendrogram by using the program START (http://outbreak.ceid.ox.ac.uk/software.htm) [28].
Multilocus linkage disequilibrium was assessed by the standardized index of association (IAS, [ 26]) using the program START version 2 [ 27].
The amplification reaction was performed in Eppendorf Master Cycle gradient AG 22331 (Model 5331), using the program that starts at 95°C for 5 min followed by 35 cycles of three steps PCR cycle at 95°C for 30 s, 54°C for 30 s, 72°C for 30 s, and final extension at 72°C for 10 min.
Since the Swift school started using the pilot program, Ms. Till said she had seen a positive shift toward more student involvement.
Also in 2010 CRWAD started using the OASIS program.
Each PSPM was developed from matches within 200 nt of the translation start site, and then used to search upstream of each gene's start codon using the FIMO program [ 20].
The sequence upstream of the precursor structure for either pre-miR168a or pre-miR168b was extracted from the NCBI database and the transcription start site (TSS) was identified by using the transcription start site identification program for plants (http://linux1.softberry.com/berry.phtml?topic=tssp&group=programs&subgroup=promoter).
Amplification was carried out using the following program: a hot start at 95°C for 4 min, followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 55°C for 60 s and extension at 72°C for 90 s, finishing with an extension at 72°C for 15 min.
They started using the program for a few months, but after the kidnapping of Peter Moore the ministry of finance immediately stopped the system.
When Copenhagen Airport starts using the program in the next few weeks, airport officials will get a real-time picture of where travelers go and what they do.
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