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The program was run using the standard settings, i.e. the scoreThresholdA was set at 10, scoreThresholdB at 0, the maximum distance between the coordinates of the putatively compatible junctions at 200 and the minimum deletion length at 500.
The isoform counts for miRNAs and mRNAs were submitted to DESeq2 (Love et al., 2014) for differential expression analysis using the standard settings.
Multiple alignments were calculated with ClustalX version 1.83.1 (ftp.embl-heidelberg.de) using the standard settings.
The programs were run using the standard settings for colorspace as recommended in the manual.
RSEM v1.1.19 [ 64] was then used to quantify mapped reads by using the standard settings.
Polar hydrogen atoms and partial charges for the protein and NADPH cofactor were calculated using the standard settings in MGLTools.
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In the search for overrepresentation we used the standard settings: Benjamini-Hochberg FDR, significance level of 0.05.
We used the standard settings for BEAGLE: 10 iterations of the phasing algorithm, drawing four samples per iteration.
The PET image reconstruction was performed with the iterative Philips reconstruction software, using a 3D line-of-response time-of-flight blob-based algorithm using the standard parameter settings (3 iterations, 33 subsets, relaxation parameter: 1.0, transverse image matrix size: 144 × 144 voxels, voxel size: 4 × 4 × 4 mm3).
Using the standard parameter settings of InfluSim, we expect only a total of 0.7% of the population to be hospitalized.
For all these samples, CNV genotypes were called using the standard PennCNV settings including adjustment for GC waves.
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using the standard tools
using the current settings
using the wrong settings
using the automatic settings
using the standard criteria
using the standard connections
using the standard data
using the identical settings
using the general settings
using the standard cut-points
using the nominal settings
using the standard drugs
using the standard arguments
using the new settings
using the standard hands
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