Furthermore, mutant mice, along with age-, sex- and genetic-background-matched controls, were analysed using the standard Sanger MGP primary phenotyping screen as described previously (see Fig. S2B; White et al., 2013).
The quality of the articles that survived this filtering was assessed by the first two authors using the Standard Quality Assessment Criteria for Evaluating Primary Research Papers [ 18].
Brains were dissected during morning hours (with genotypes in random order) from 0- to 7-day-old adult flies and stained with primary antibodies using the standard protocol described in Soldano et al (2013).
In this pragmatic trial, patients will undergo routine elective primary unilateral TKA using the standard technique of the anaesthetist and the operating surgeon.
We assumed that the standard deviation of timber volume, S h, would be unknown for the purposes of survey design and used the standard deviation of the primary stratification variable instead.
There are many advantages for pregnant women, hospital staff and primary care staff with this model, which may also prove more cost-effective than the standard primary care model currently used in South Africa.
All studies reporting the primary outcome used the standard dose recommended by WHO (table 2), except for one.
Wild-type (WT) or mutant CRT proteins were immobilized on a biosensor chip at about 500 RU using a standard primary amine coupling method.
Imaginal discs were fixed and stained by standard procedures using the following primary antibodies/dilutions/sources: anti-Serrate (1 100), anti-β-galactosidase (1 500; Cappel), anti-cleaved Caspase-3 (1 200; Cell Signaling Technologies), 4D4 (anti-Wg; 1 100), 4F3 (anti-Arm; 1 100), and anti-Elav (1 50; all from the Developmental Studies Hybridoma Bank).
Immunostaining was performed with standard protocols using the following primary antibodies: human NANOG (rabbit polyclonal, Abcam, 1 500), TRA1-81-Alexa-Fluor-488-conjugated (mouse monoclonal, BD Pharmingen, 1 20), RYR2 (mouse monoclonal, Thermo Scientific, 4 µg/ml), MLC2a (mouse monoclonal, Synaptic Systems, 5 µg/ml) and MLC2v (mouse monoclonal, Synaptic Systems, 5 µg/ml).
