Sentence examples for using the standard preparation from inspiring English sources
Our study 12 was a pragmatic randomised trial 13 designed to compare the two procedures undertaken by doctors or nurses, with or without sedation, using the standard preparation, techniques, and protocols of participating hospitals.
One single-stranded library using the standard library preparation kit and two double-stranded libraries using a rapid library preparation kit with an average insert size of 705 bases were used for sequencing.
These were quantified by Western blotting and by cytometry using the standard cell preparations.
Transfection of ARRDC3 promoter was conducted into primary cells on the 7th day of culture using the standard calcium-phosphate preparation.
For PacBio RS sequencing, two types of SMRTbell DNA template libraries were created with 1- and 10-kb sheared genomic DNA, and prepared using the standard PacBio RS sample preparation methods with C2 chemistry specific to each insert size.
The ZO-1 and GAPDH RNAs used in the standard preparation were 327 bp and 335 bp long, respectively.
Sequencing libraries for all isolates were constructed using the standard genomic DNA sample preparation kits from Illumina (Illumina, Inc, San Diego, CA), according to manufacturer's instructions.
Our initial attempts using the standard 100°C denaturation in preparation for running an SDS-PAGE resulted in protein cleavage as others have also observed for cipA[ 36, 37].
We have carried out these experiments using the standard approach for slice preparations, as described by Studer et al., although we used 6 week old animals, and not 'pups' (Studer et al., 2014).
After fragmenting of the cRNA for target preparation using the standard Affymetrix protocol, 15 µg fragmented cRNA were hybridized for 16 h at 45°C to Mouse Genome 430 Version 2.0 Array (Affymetrix) which includes 45101 probe sets.
The small RNA samples were then separately committed to strand-specific sequencing library preparation using the standard Illumina TruSeq Small RNA kit.
