All genotyping was conducted on the 7500 Fast real-time PCR system using the standard run mode, and the genotypes were generated using the 7500 Fast system SDS software version 2.0.6 (Life Technologies).
Firstly, the GaAs buffer layer (of about 100 nm) and the first AlAs barrier were grown using the standard MBE growth mode at the high temperature of 600 °C.
Both the operated and unoperated hips were scanned simultaneously using the standard dual femur acquisition mode of the instrument.
The platform was operated in the nano-LC mode using the standard nano-ESI API source fitted with a PicoTip emitter that had an uncoated fitting and 10-µm spray orifice (New Objective, Inc., Woburn, MA).
The platform was operated in the nano-LC mode using the standard nano-ESI API stack fitted with a picotip emitter (uncoated fitting, 10 µm spray orifice, New Objective Inc., Woburn, MA).
The PCRs were performed on an Applied Biosystems 7500 Fast Real-Time PCR System in Fast 7500 run mode using the standard thermal cycler protocol.
The platform operated in the nano-LC mode using the standard nano-ESI API stack fitted with a picotip emitter (uncoated fitting, 10-μm spray orifice, New Objective, Inc., Woburn, MA, USA).
The platform operated in the nano-LC mode using the standard nano-ESI API stack fitted with a picotip emitter (uncoated fitting, 10 µm spray orifice, New Objective, Inc., Woburn, MA).
The particles' velocities are calculated using the established relationships in the standard mode.
