qRT PCR was performed on the StepOne Real-Time PCR System (Applied Biosystems) using the standard instrument protocol for the TaqMan Protein Assay.
The historical scenario, in which all the patients (N = 139) were treated using the standard reusable instrument kit, was compared with the innovative scenario, thus considering 119 procedures performed with the reusable instrument kit and 20 with the innovative disposable one.
Study participants were questioned about the prevalence of diarrhea in the household in the past two weeks using the standard CDC instrument.
Using the standard outcome instrument, Western Ontario and McMaster Universities' Osteoarthritis Index (WOMAC) for pain and function scores, independent assessors unaware of treatment assignment performed outcome assessments at weeks 3, 6, 12, and 24 after the initial treatment.
These were: age, gender, skin color (white/non white), marital status (living with or without a partner), monthly income (categories of multiples of the official minimum wage), years of schooling, smoking (never smokers; ex-smokers; current smokers), alcoholism (using the standard CAGE instrument)[ 30] and economic level.
For insertion of the DLC cup in the trapezium, a spherical drill was used to drill a cavity formed to the measured size of the cemented DLC cup using the standard DLC measuring instruments, and the cups were fixated with Palacos gentamycin bone cement (Haereus Medical, Hanau, Germany).
A dissociation curve analysis was run using the standard protocol on the instrument.
The coloration of the two types of mealworm was recorded using a Minolta CM-2600d specthathotomeasureshat measures from 360 to 700 nm and provides values of brightness, chroma and hue (LCH) using the standard software provided by the instrument [20].
Quantitative morphometric analysis of the images was performed using the standard software provided with the instrument.
Sequencing was performed on a GS FLX instrument, using the Standard LR70 Sequencing Kit (Roche).
