Sentence examples for using the standard assay from inspiring English sources
The initial velocity of the reductive reaction was analyzed using the standard assay conditions.
Kinetic parameters (Km and Vmax) were determined by the measurement of activity against pNPX using different substrate concentrations (0.5 - 12 mM) using the standard assay procedure.
After the enzyme (in 10 mM potassium phosphate buffer, pH 7.2) was incubated for 30 min at each temperature, the residual activity was determined using the standard assay at 50°C.
Strain MW1001 produces no H2 during anaerobic growth, and cell extracts have no detectable hydrogenase activity at either 37 or 80°C (using the standard assay of methyl viologen (MV -linked H2 evolution vide infra).
The residual activity was measured using the standard assay.
The residual xylanase activity was determined using the standard assay.
When screened using the standard assays described in Methods, MW150 exhibited a T1/2 > 60 min for all of the recommended CYPs.
Osmotic avoidance behavior was assayed using the standard drop assay [ 23]. C. elegans responds within a second of drop delivery by backing away from the drop of osmotic solution [ 23].
Protein concentrations were determined by assaying samples using the standard Bradford assay (Bradford, 1976).
This was confirmed in the present study using the standard MTT assay, the lactate dehydrogenase (LDH) release assay, and the colony formation efficiency (CFE) assay, and CuOOH was therefore used as a positive control in all subsequent experiments (4).
Cell proliferation was determined using the standard MTT assay [ 19] and the phosphatase activity assay [ 20].
