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Meanwhile, only 3% share using the standalone share button.
Computational predictions of localization were performed using the standalone version of PSORTb v.2.0 [ 31].
Protein family analyses were done using the standalone versions of PANTHER [ 67].
Sequences were aligned and their pairwise divergence calculated using the standalone version of BlastAlign (Belshaw and Katzourakis 2005).
It operates by querying SaddleSum locally (using the standalone version) or remotely (through an HTTP request to a web server).
For batch mode analysis, prefer to use the standalone GUI version.
To predict signal peptide cut sites, we used the standalone version of SignalP 3.0 [ 19, 36, 20].
To identify the putative new miRNA targets we used the standalone-based UEA sRNA toolkit-Plant target prediction pipeline.
using the NCBI standalone BLAST program.
The ARACNE network was constructed using the aracne2 standalone software package according to authors instructions [ 40].
The expression of known miRNAs and other transcribed sequences was analysed using the miRanalyzer standalone version.
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