Meanwhile, only 3% share using the standalone share button.
Computational predictions of localization were performed using the standalone version of PSORTb v.2.0 [ 31].
Protein family analyses were done using the standalone versions of PANTHER [ 67].
Sequences were aligned and their pairwise divergence calculated using the standalone version of BlastAlign (Belshaw and Katzourakis 2005).
It operates by querying SaddleSum locally (using the standalone version) or remotely (through an HTTP request to a web server).
tRNAs were annotated using the standalone version of tRNAscan-SE v1.3.1 (Lowe and Eddy 1997) (COVE-only) and checked using TFAM v1.4 (Tåquist et al. 2007).
For batch mode analysis, prefer to use the standalone GUI version.
To predict signal peptide cut sites, we used the standalone version of SignalP 3.0 [ 19, 36, 20].
To identify the putative new miRNA targets we used the standalone-based UEA sRNA toolkit-Plant target prediction pipeline.
using the NCBI standalone BLAST program.
Using this sequence information, probe sequences were matched at the probe level by mapping Affymetrix short oligonucleotides to porcine spotted glass long oligonucleotide sequences using the BLAST standalone program as described by Kuo et al. [ 27].
