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The density of cells on the particulate material remaining on the filter was then determined using the staining method described above.
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In order to detect the expression of LMP1 and histone H3 phosphorylation at Ser10 in CNE1G and CNE1GL cells, they were immunohistochemically stained using the same staining method as for the clinical specimens.
In brief, AChE activity staining was conducted using the Karnovsky Roots staining method (Karnovsky and Roots 1964; Kaufer et al. 1998).
3-Nitrotyrosine level in cultured cells was determined using the immunocytochemical staining method.
Reticulocyte counts were determined using the supravital staining method [ 13].
We measured infarction volume using the TTC staining method.
The enzymes appeared to be homogeneous on the basis of SDS−PAGE developed using the silver staining method.
We studied the effect of curcumin, NDGA or MK886 on apoptosis in MCF-7 cells using the Hoechst staining method.
By using the SYPRO Ruby staining method, more than 2,000 protein spots were detected on each 2-D gel.
One phenotypic change in the evolving chemostat population was detected by using the iodine staining method as described previously in Notley and Ferenci (1995).
The amount of biofilm formed was evaluated based on the biofilm coverage rates (BCRs) before and after VCM administration, bacterial viability in biofilm was visually observed using the fluorescence staining method, and the viable bacterial count in biofilm was measured.
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