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As shown in Figure 6C, we observed results similar to those from the in vitro migration experiments carried out using the stable cells.
To determine whether mutation of NLS of IGFBP5 affects cell motility, we performed an in vitro migration assay using the stable cells overexpressing either wild-type or mutant IGFBP5.
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Using the stable cell lines not only provides an optimized solution for manufacture of therapeutic and diagnostic protein, but also for applications in drug screening, pharmacological research, and toxicological studies.
From the data shown in our studies using the stable cell lines, overexpression of miR-31 alone seems to be sufficient to induce apoptosis in WPE1-NB26 cells at comparable level to WPE1-NA22 cells.
Finally, to test if endogenous FoxO3 is methylated in cells by endogenous Set9, we used the stable cell line with Set9 knock-down.
Consistent with our finding using the stable knockdown cells, we observed that the release of HCV Core or the infectivity of HCV into the culture supernatants was significantly suppressed in these transient knockdown cells 24 hrs after HCV-JFH1 infection (Fig. 1L and 1M).
Using the stable ARE luciferase reporter cell line derived from MDA-MB-231 cells combined with a 96-well high-throughput screening system established in our laboratory, we identified a novel Nrf2 activator that belongs to the class of diterpenoids.
The proteome analyses were performed on two EBV-transformed lymphoblastoid cell lines derived from the two patients using the stable isotope labeling in cell culture (SILAC) technique for accurate quantification (Mann, 2006).
In vitro analyses with Western blotting and semiquantitative reverse transcription-polymerase chain reaction were performed using the stable SNU-638 gastric cancer cell line transfected with lentivirus-delivered FOXO1 short hairpin RNA.
To assess whether FXYD6 was involved in the tumor formation and growth in vivo, we established xenografted HCC models in nude mice using the stable transfectants of SMMC7721-mock and SMMC7721-FXYD6 cells (Fig. 5A).
To investigate the role of chaperone Lon in cell survival under environmental stresses, we used the stable 293 cells overexpressing Lon (293/Lon) to identify the associated proteins of chaperone Lon by proteomic approach, by which we could exclude most substrates of Lon protease.
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