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Using the SSR marker orders in the framework map as the preferred orders, 47 markers composed of 36 SSR and 11 SRAP markers were identified in the maternal map.
Hybrids were identified using the SSR marker Ah-282 visualized in 3% agarose gels.
A total of 87 recombinants were found at border B from initial screening using the SSR marker P3K2608.
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Using the SSR markers a genetic map can be established, the available sequence extensions by the underlying ESTs will greatly facilitate the implementation of the SSR marker loci during the pending iterative assembly process of the Physcomitrella genome sequence data.
Furthermore, the genetic distance among the parents was determined using the SSR and RAPD molecular markers.
On the contrary, none of these crosses were predicted by the genetic distance as it was calculated using the SSR and RAPD molecular markers.
For the clarification of this problem field experiments were conducted for four consecutive years to compare the effectiveness of six criteria for the prediction of the most promising F3 populations in durum wheat (Triticum durum L .: the mid-parent value, the F1, the F2, the (F1 + F2)/2, and the genetic distance among the parents as it is calculated using the SSR and RAPD molecular markers.
Using only the SSR markers, we built a composite map covering 709 cM.
Methods and Results: Eleven primer pairs were developed using the compound SSR marker technique.
Using the common SSR markers as anchor markers, comparative analysis identified the collinearity of these linkage maps.
The linkage map for the F2 population derived from TARI-08874 and 'Bai-li-gua' was constructed using the polymorphic SSR markers and the R statistical software (R Core Team 2013) with the qtl package (Broman et al. 2003).
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