Sentence examples for using the ssr enrichment from inspiring English sources

Microsatellite markers in cultivated peanut were developed using the SSR enrichment procedure.

Furthermore, the genetic distance among the parents was determined using the SSR and RAPD molecular markers.

The number of potential EST-SSRs per unigene varied from 1 to 8, with an average of 1.25.> Using the SSR-containing sequences, 113 SSR sites were randomly selected to design EST-SSR primers with the Primer Premier 3.0 software.

So far, the first two approaches have been used for developing SSR markers in pigeonpea with some success despite the labour-intensive and time consuming nature of the SSR enrichment and very low polymorphism levels of SSRs identified from the mining of transcript sequences.

When microsatellites are isolated using standard SSR enrichment protocols and primers designed at complementary repeated regions, amplification from multiple genomic sites can cause scoring difficulties that compromise their utility as markers.

SSR polymorphism: A total of 3160 g-SSR primer pairs from the SSR enrichments, 50 newly developed e-SSRs, and 351 publicly available e-SSRs were used to screen for polymorphisms in the parents of the KH and CK populations.

This may be attributed to the size range of insert, the restriction enzyme used for genomic DNA library construction and the approach used for SSR enrichment, etc. [ 7].

Enrichment analysis was conducted using the Singular Enrichment Analysis (SEA) tool in the agriGO toolkit [ 84].

'Central bins' were within the enrichment zone as defined using the heuristic enrichment thresholds described above.

Gene enrichment analysis was performed using the GO enrichment analysis tool in AmiGO [ 55].

The significance of enrichment was calculated using the Gene Set Enrichment Analysis (GSEA) software [ 12].