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In the present study, profiles were obtained by ultra-deep sequencing using the SOLiD platform (Life Technologies, CA, US).
Using the SOLiD platform, we generated approximately 62 million 25 bp reads (Table 1).
The extracted RNAs were labeled in a strand-specific manner and sequenced using the SOLiD platform.
The six libraries were sequenced using the SOLiD platform, resulting in 10 453 genes.
The whole-transcriptome sequencing of HepG2 cells was performed using the SOLiD platform and validated using droplet digital PCR.
We constructed five small RNAs libraries from sorghum stem tissue at the time of flowering and sequenced them using the SOLiD platform.
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arietinum ICC4958 and C. reticulatum PI489777) were also prepared by the SOLiD Opti Mate-paired library kit following the manufacturer's protocol and sequenced using the SOLiD 4.0 platform (Applied Biosystems, Foster City, CA, USA).
Another critical point was highlighted by our results from analysis of RNA-seq reads obtained using the SOLiD 3 platform: the SOLiD analysis software removes the reads partially or completely overlapped to the poly-A.
arietinum ICC4958 and C. reticulatum PI489777) were sequenced using the SOLiD (Applied Biosystems) platform to generate 28 Gb (37.8X) and 12 Gb (16.2X) high-quality paired short reads (50 bases), respectively.
For this purpose, and to avoid tissue specificity, we subjected mixture of RNA samples from four tissues (brain, lungs, muscle, and heart) extracted from a single C. chamaeleon specimen to MPS using the SOLiD ABI platform.
Deep sequencing of small RNAs from SS patients and healthy controls using the SOLiD 4 platform resulted in the discovery of six novel miRNAs including hsa-miR-4524b-3p, hsa-miR-4524b-5p, hsa-miR-5571-3p, hsa-miR-5571-5p, hsa-miR-5100, and hsa-miR-5572 hsa-miR-5572 hsa-miR-5572
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