Sentence examples for using the solid mate-pair from inspiring English sources

One mate-pair library (1.5 – 3.5 kb) for each strain was constructed using the SOLiD mate-pair library kit and sequenced on a SOLiD4 Sequencer (Life Technologies).

Mate pair libraries were generated as per the SOLiD Mate Pair Library preparation protocol.

To investigate the performance of SOLiD sequencing in a metagenomic context, we compared taxonomic profiles of SOLiD mate-pair sequencing reads with Sanger paired reads and 454 single reads.

The genomic DNA was then extracted by QIAamp DNA Mini Kit (Qiagen, Valencia, CA, USA) and subjected to SOLiD mate-pair library construction.

Thus, SOLiD mate-pair sequencing seems to be a viable and cost-efficient option for analyzing a complex environment such as the human intestinal microbiome.

This study shows that SOLiD mate-pair sequencing is a viable and cost-efficient option for analyzing a complex microbiome.

> -wrap-foot> scaffoldingscaffofding of the 1 08563-contigig Newbler output was performed using the Illumina mate-pair information.

Genomic DNA from a Black Angus bull and a Holstein bull were sequenced using the SOLiD system and a combination of fragment and mate pair libraries (Table 1).

arietinum ICC4958 and C. reticulatum PI489777) were also prepared by the SOLiD Opti Mate-paired library kit following the manufacturer's protocol and sequenced using the SOLiD 4.0 platform (Applied Biosystems, Foster City, CA, USA).

Super-scaffolding was performed in order to merge the existing gap-closed scaffolds into super-scaffolds using relative orientation of SOLiD mate pair reads.

The scaffolds were then further ordered and linked using the mate-pair libraries, estimating gaps between the contigs according to the distance of mate-pair links.