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A three factor, three-Level Box Behnken design was used for the optimization procedure, using the software Design Expert V 7.1.1.
A three factors, three levels Box-Behnken factorial design was used for fitting a second order response surface, using the software Design Expert 7.1.1 (Stat-Ease, Minneapolis, MN, USA).
The experimental design was performed by using the software Design Expert 7 (Stat-Ease, Minneapolis, MN, USA).
The analysis of variance (ANOVA) and the graphical analysis of the data were also performed by using the software Design Expert 7 (Stat-Ease, Minneapolis, MN, USA).
Statistical analysis of the results was performed using the software Design Expert 7.0 (trial version), and response surface graphics were plotted with STATISTICA 7.0 (trial version).
The graphical representation of the regression equations called the response surfaces and 3D curves were obtained using the software Design Expert 7.1.2 and were presented in Figure 7.
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The value was close to 98.25%, which was the optimal result predicted by response surface methodology using the software Design-Expert 8.05b.
With 145 cases of FEM calculations being the samples, a response surface model (RS M is developed to predict the pipeline lateral displacement using the software Design-Expert.
For five consecutive weeks experimental and control group students used the software designed for this research.
Contigs from these assemblies were used for primer design using the software PrimerExpress (Applied Biosystems).
Primers flanking the indels were designed using the software Primer Premier 5.0 and tested on the parents.
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