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The percentage of GFP-expressing cells was quantified using the software analysis.
Time-lapse imaging series were acquired using the software analySiS from Soft Imaging System with a ColorView-12 digital colour camera.
Fold changes (ΔCt) were calculated in the following manner: the cycle number (Ct) of the target genes were extrapolated using the software analysis program (SDS 1.9, Applied Biosystems) and was subtracted from the Ct of the input control.
MiRNA target prediction was performed using the software analysis tools included into the following websites: http://gencomp.bio.unipd.it/magia/start (Magia), http://www.targetscan.org (targetscan) and http://diana.cslab.ece.ntua.gr/pathways (Diana Lab).
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To determine the density of black dots per unit cytoplasmic area, we used the black and white images and the command 'phase analysis' using the software analySIS® with similar threshold values.
Using the software, forecast analysis for the re-frozen of Feng Huoshan permafrost Tunnel is made.
All experimental data were performed in triplicate and processed by two-way ANOVA using the software Statistical Analysis System (SAS).
Peaklists of MS/MS spectra were generated using the software Data-Analysis 3.3 with default parameters.
Data analysis was performed using the software Comprehensive Meta-Analysis version 2.0.
Multimarker LD analysis was done using the software MIDAS (Multiallelic Interallelic Disequilibrium Analysis Software) [ 28].
We performed this analysis using the software PAML [38].
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