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The alignment was done using the software T-Coffee [35].
All of the stem-loop sequences of the MIR159 family were aligned using the software T-coffee version 6.06 [ 68].
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The sequences were aligned using the program T-coffee (EBI) and formatted with ESPRIPT (Gouet et al., 1999 ).
12S and 16S ribosomal RNA genes were aligned using the program T-COFFEE [ 24] incorporating information from secondary structure [ 25].
The predicted proteins of the planarian MEX3 homologs were aligned using the program T-coffee along with MEX3 homologs from other species (Notredame et al., 2000).
CTD+ domain sequences were aligned with MAFFT L-INSI [ 66], ProbCons, Muscle and T-coffee and subsequently combined into an optimal alignment using the combiner function of T-coffee [ 67].
Calculations of sequence similarity and the informativeness of sequences was done using the T-Coffee package [43].
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The orthologous regions were realigned using the T-coffee algorithm, and then submitted to Phylogibbs.
Protein alignments were created using the T-COFFEE algorithm and EST searches conducted using UniGene.
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