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RM and AW assessed the characteristics using the SMART criteria.
Each characteristic was reviewed using the SMART criteria by two authors (RM and AW).
Each characteristic was assessed by two of the authors using the SMART criteria and initial agreement was reached for 80% of characteristics.
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In cooperation with the patient, the APN explores the motivation for change, gathering goals for the patients using the SMART-criteria [ 23]: Specific, Measurable, Achievable, Realistic/ Relevant, Timed.
These sequences were then verified using the SMART tool (http://smart.embl-heidelberg.de/) [ 37].
Protein domain boundaries were identified using the SMART protein prediction database (http://smart.embl-heidelberg.de/).
Full-length cDNAs were prepared using the SMART technology [ 35].
Structural motif annotation was performed using the SMART http://smart.embl-heidelberg.de[ 95] and Pfam http://pfam.sanger.ac.uk databases [ 96].
This RNA pool was then used to prepare cDNA using the SMART protocol (as above).
Using the SMART software (http://smart.embl-heidelberg.de/smart/show_motifs.pl), a phospholipase C catalytic domain (noted 'PLCXc') was detected in the XP_293875 protein sequence associated to BX648329.1 mRNA.
Double-stranded (ds) cDNA and RACE template synthesis was performed using the SMART PCR cDNA Synthesis kit and SMART RACE cDNA Amplification Kit (Clontech).
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