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Plasmid was generated in Top 10 cells (Invitrogen) and the plasmid was purified using the Small Scale Plasmid DNA Purification Kit (QIAGEN).
In addition, we describe a refinement using the small scale transfection of HEK293 cells which facilitates the identification of functional antibody expression plasmids.
The GST-tagged SEpHluorinA277D was isolated using the BugBuster GST-Bind Purification Kit (Novagen 79794 3 REF) and purified using the small scale batch method according to the manufacturer's instructions.
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The experiment is carried out using the small-scale Darrieus wind turbine in a water tunnel.
The PMMA/CNF composites were processed into 4 mm diameter rods and 60 μm diameter fibers using the small-scale melt processing fiber spinning equipment.
For the large-scale radial flow capsule, the CFD model quantitatively predicts breakthrough data using binding parameters independently determined using the small-scale axial flow capsule, identifying structural irregularities within the membrane pleats as an important source of band broadening.
We used the smallest scale of census data available to account for area-level maternal education and income, which attenuated the association between greenness and birth outcomes.
Here, we use the small-scale properties of the core promoter that have been described, but then not used for promoter prediction.
While web-based predictions are easy to use on the small scale, it severely hampers large-scale predictions and makes a direct comparison of individual methods difficult.
Approaches to ecological small-area analysis propose using the smallest practicable spatial scale for any study on inequalities in health [ 8, 9].
The FunCoup tool (www.funcoup.sbc.su.se) was used to build the small scale networks.
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