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After a CT scan of the head, SPECT scan was performed using the small animal FLEX system equipped with multiple pinhole collimators (1 mm) from 15 to 57 min post-injection of 99mTc-HMPAO, since radioactivity concentration in the brain is steady from 14 s to 60 min post-injection of 99mTc-HMPAO [3].
using the small animal software application.
Adenomas were grossly imaged using the small animal endoscope to view binding to the adenoma as a whole.
A list-mode SPECT scan for a Tc99m filled small sphere, embedded in a water-filled rat-sized cylinder, was acquired using the small animal scanner.
LUS images were obtained using the small animal ultrasound, an animal-dedicated machine (VisualSonics Vevo® 2100 System; VisualSonics Inc ,Toronto, Ontario, Canada) with the cardiac probe (30 MHz).
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Moreover, our previous articles showed the solidity of the data which were obtained using the small-animal PET/SPECT/CT system [25] and the utility of the PET/SPECT analysis in the pre-clinical study for the cell therapy against stroke [24, 42].
Analysis was performed using the small-animal mode of the encore software (GE Healthcare, version 13.40) and was calibrated at each start of the experiment.
Briefly, all mice (including mice receiving sham radiation) were anaesthetized by intra-peritoneal (i.p.) injection of avertin (240 mg kg−1) and the primary tumours irradiated with a single fraction of 8 Gy, 20 Gy or 30 Gy on day 12 or with 3 fractions of 8 Gy on day 12, 13 and 14 using the Small Animal Radiation Research Platform (SARRP Xstrahl, Surrey, UK).
For in vivo experiments, mice were treated using the Small Animal Radiation Research Platform (Wong et al, 2008).
The PET/CT images were obtained using the Inveon small animal imaging system (Siemens Medical Solutions, Knoxville, TN, USA).
PA measurements were performed using the Imagio small animal PA imaging device (Seno Medical Instruments Inc., San Antonio, TX) shown in Fig. 1.
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