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The revertants were checked for DNA differences using the simple sequence repeat technique.
Three genotypes of the Hwayeongbyeo homozygous and heterozygous and O. rufipogon homozygous classes were identified using the simple sequence repeat marker RM194.
Genotyping was performed using the simple sequence length polymorphisms (SSLP) listed on the ZFIN web site.
Simple sequence repeats (SSRs) present in the EST sequences were identified and analyzed using the simple sequence repeat identification Tool (SSRIT) [ 34].
The repetitive regions were identified using the Simple Sequence Repeat Identification Tool (Temnykh et al., 2001), and 30 primer pairs were designed using WebSat (Martins et al., 2009).
Using the Simple Sequence Repeat Identification Tool (SSRIT, http://www.gramene.org/db/markers/ssrtool), a total of 39,257 potential simple sequence repeat (SSR) were identified in 16,208 unigenes.
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Besides conventional approaches, we have used the simple sequence repeat (SSR) signatures as one of the prediction parameter.
To locate and characterize SSRs in these genomes, we used the Simple Sequence Repeat (SSR) Extractor Utility [ 80].
In order to get more information on the hyperpolarization process, hyperpolarized high-resolution ULF spectra were additionally acquired using the simple FID sequence.
Our analysis of a large number of clinical isolates over 20 years reinforces the pertinence of this technique that allows the identification of most HEV sequences using the simple computationally non-intensive genetic distance calculation.
We evaluated the reliability and performance characteristics of CNV detection using the simple analysis based on the deep sequencing data, as described above, by comparing the results with those obtained from high-density exon-targeted aCGH.
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