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YoungThing shoots, using the silencer.
He remembers his minder impressing on him the importance of not using his weapon unless it is imperative or in self-defense; and of using the silencer if he does have to shoot.
Validated siRNA for SIRT1 (Santa Cruz Biotechnology) and the appropriate control RNAi (Applied Biosystems) were transfected into WI-38 cells using the Silencer siRNA Transfection II Kit (Applied Biosystems) according to the protocols provided by the manufacturer.
Small interfering RNAs (siRNAs) targeting Su var)205 and Dnmt2 were designed using the "siRNA at Whitehead" [57] generated by in vitro transcription using the silencer siRNA construction kit (Ambion).
The CTCF siRNA (Santa Cruz Biotechnology) was made into 10 µM stock using nuclease free water and 9 nM siRNA was delivered to the cells using the Silencer siRNA Transfection kit (Ambion/Applied Biosystems, TX, USA) according to the manufacturer's instructions.
DsRNA was generated using the Silencer® siRNA Construction Kit (Ambion).
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The trust would also allow his fiancée to use the silencers once the couple married.
But authorities never presented evidence to suggest Dorner had used the silencers during his rampage.
To be able to track dsRNA during tick feeding, tick β-actin dsRNA was fluorescently labeled using the Cy™3 Silencer siRNA labeling kit (Ambion) with minor modifications to the manufacturer's protocol.
To generate siRNAs, oligonucleotides for sense and antisense-strands of each siRNA together with T7 promoter sites were synthesized and siRNAs were produced using the Ambion Silencer siRNA construction kit (Ambion).
CYP2E1 short hairpin RNA (shRNA) P-silencer was synthesised using the P-silencer 2.1-U6 hygro vector (Agilent Technologies, Wokingham, UK).
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