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The assay was quantified on a PerkinElmer FusionAlpha plate reader, triplicate values were averaged, and titration curves (three-parameter sigmoidal fit) were plotted using the Sigmaplot graphing program.
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The statistical analysis was performed using the SigmaPlot software (V 12.0; Systat Software, Inc ., whereas the graphs were made with GraphPad Prism® software (V 6.01; GraphPad Software , Inc.
The statistical analysis was performed using the SigmaPlot software 10.0.
All the statistical analyses were conducted using the SigmaPlot for Social Sciences version 13.
Figures were created using the SigmaPlot software (SigmaPlot 10.0) with help of Adobe Photoshop CS3 and Adobe Illustrator CS3.
Histograms were adjusted to a Gaussian curve using the Sigmaplot software.
Analyses were performed using the Sigmaplot software.
Quadratic regression fits were implemented using the SigmaPlot software package.
Statistical analysis was performed using the SigmaPlot software (Systat Software, San Jose, CA).
Statistical analysis was performed using the Sigmaplot v.11.0 software for Windows.
All statistical calculations and graphs were made using the software SigmaPlot (version 12.0).
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