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A verification of these analyses was made using the SigmaPlot (Addlink, Barcelona, Spain) graphics package that was also used to plot the positive and negative known status sera EI histogram and to calculate the 95% sensitivity and specificity confidence intervals at each cut-off.
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The statistical analysis was performed using the SigmaPlot software 10.0.
All the statistical analyses were conducted using the SigmaPlot for Social Sciences version 13.
The mean value and standard deviation [SD] were calculated using the Sigmaplot 10.0 (Systat Software, Inc., Chicago, IL, USA).
Figures were created using the SigmaPlot software (SigmaPlot 10.0) with help of Adobe Photoshop CS3 and Adobe Illustrator CS3.
Histograms were adjusted to a Gaussian curve using the Sigmaplot software.
Statistical differences between experimental conditions were analyzed by a t-test or paired t-test using the Sigmaplot 9.0 software.
Analyses were performed using the Sigmaplot software.
Quadratic regression fits were implemented using the SigmaPlot software package.
Statistical analysis was performed using the SigmaPlot software (Systat Software, San Jose, CA).
Statistical analysis was performed using the Sigmaplot v.11.0 software for Windows.
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