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Tolerance scores were generated using the SIFT program and entering in the NCBI protein G numbers for each M. musculus gene at Skts5 [ 29].
This finding was supported using the SIFT program which predicts the functional relevance of SNPs by comparison of conservation at that site [ 27].
The effects of ns substitutions on a given protein were evaluated on a local server using the SIFT program which predicts the effects of missense substitutions on a protein based on evolutionary information from homologous protein sequences.
Once the above amino acid changes in Leishmania spp. were determined, we carried out an analysis of tolerance prediction of the AAS on the colchicine-binding region of bovine, which was used as reference organism, using the SIFT program (see Section 2).
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First, we find the paired matching pixel points in the two corresponding digital images by using the SIFT matching algorithm.
Variant functional impacts were predicted using the SiFT Blink tool (http://sift.jcvi.org/www/SIFT_BLink_submit.html) (Kumar et al. 2009).
The deleterious effect of non-synonymous SNPs were analysed using the SIFT and PolyPhen algorithms.
A possible effect of the validated SNPs was analyzed using the SIFT and CFSSP prediction tools.
Using the SIFT tool, the result was "Damaging" to protein function [ 11].
The algorithms for the SIFT program use SWISSPROT, nr, and TrEMBL databases to find homologous sequences.
We used the SIFT, Poly Phen and PANTHER programs to predict the influence of thousands of amino acid-substituting CVs on protein function.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com