DNA was then extracted from a swab of the lesion and two sets of 'outward facing' primers were designed using the short sequences to amplify the entire 7600 bp genome of the novel PV.
It is uncertain how much of this phenomenon is due to a "principle of least effort," but using the shortest sequences for the most common words certainly promotes greater communication efficiency.
The multilocus sequence type (MLST) genotype of the strains were determined from sequencing read data using the short read sequence typing (SRST) tool (Inouye et al. 2012).
A separate measure, α*, was calculated using the short intron sequence class as our neutral standard.
Genomic sequencing was performed using genomic DNA prepared by standard methods (Sherman et al. 1987) using the short read Solexa sequencing technology from Illumina (www.illumina.com) (Bentley 2006).
However, in contrast with direct sequence analysis of the NS5B gene, which uses a long nucleotide sequence, the real-time PCR assay uses only the short sequence of its primers and probes.
For such regions, the identification of mutations is difficult by using the short-read sequencing technology.
In 2004, Takahara et al. reported a unique concept of whole-body DWI using the short tau inversion recovery (STIR -EPI STIR -EPIand free breathing sequence (diffusion-weighted whole-body imandng with background body signal suppression: DWIBS).
In order to make the best use of the short sequence reads for SNVs (Single variants: short INDELs and SNPs) discovery, processed SOLiD reads were aligned to this available melon genome assembly (v3.5) [ 36].
