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By using the settings stated above we attempted to ensure all primers would amplify with a Polymerase Chain Reaction (PCR) annealing temperature of 56°C.
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The scanning protocol consisted of 17 volumetric acquisitions with identical intervals in both groups obtained over a period of 66 s using the tube settings stated above.
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We inferred haplotype phases for the reference data using the settings --rounds 50 --states 200.
In the first stage, haplotype phases for the reference data were inferred using the settings -rounds 50 -states 200.
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In order to determine the percent of the genome that was mapped by uniquely mapped read, we used the previously stated settings with nonspecific reads being ignored.
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