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Using the SOLiD V3.0, 50 bp sequencing reads were generated for each sample and resulting high quality reads were compared/aligned to the existing genome sequences of the H. influenzae strains Rd KW20, 86-028NP and 10810 to determine sequence homology using the SETS software tool that is integrated into the SOLiD platform.
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We required all added protein-protein interactions between these two networks to be in the Ingenuity Expert Findings and Ingenuity ExpertAssist Findings, and further required that the interaction had been verified in human lungs by using the setting within the IPA software.
Using the set color threshold tool of Metamorph software, we established a 174-255 HUE range to select and quantify the area occupied by crystall violet-stained cells.
The presence of individual bands in each profile was assessed using the same software, set to a 1% tolerance of band matching.
To test this hypothesis, we carried out GO analysis of functional categories that are enriched in this commonly responsive gene set, using the Pathway Studio software package.
Neighbour-joining trees were constructed with 1000 bootstrap sets using the Mega4 software package [ 84].
We performed postprocessing of the MRS imaging data sets using the Functool software package version 2.2.49 (General Electric Company).
To address the ability of these self-corrected reads to be assembled, we self-corrected both the 110× and 185× BL21(data data sets using the PacBio software package.
For 76 pulmonary nodules identified on these scans, volumetric measurements were obtained on all three CT data sets using the GE Volume Analysis software.
Gene sets enrichment analyses were performed using the GSEA software v2.04 and gene sets from the MSigDB available at www.broad.mit.edu/gsea (permutation type gene sets, 1000 permutations).
After specifying levels for each parameter (factor), design of experiment is performed using the Minitab software to set these two groups of parameters (Figs. 1, 2, 3).
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