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They were searched against databases for protein identification using the Sequest software.
The peak list files generated by the Bioworks software (Applied Biosystems, USA) using the default parameters were searched against databases for protein identification using the Sequest software.
The mass spectrometry data was analyzed and compared using the Sequest software (Bioworks3.31, Thermo) searching the mouse section of the NR-NCBI database.
The mass spectrometry data was clustered and analyzed by using the Sequest software [15] and Pep-Miner [16] searching engine against the Human part of the NR-NCBI database.
The MS/MS spectra were extracted from the mzXML file using the program MzXML2Search and all data was processed using the SEQUEST software.
Sequencing data generated from the ion trap was analyzed using the Sequest software and a human database subset created from NCBI non-redundant data (68).
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The mass spectrometry data were analyzed using the Sequest 3.31 software (J Eng and J Yates, University of Washington, Seattle, WA, USA, and Finnigan, San Jose, CA, USA) and Pep-Miner searching against the human part of the NCBI-NR database.
Data were searched against the Danio rerio IPI database v3.45 using the SEQUEST algorithm in the BioWorks software program version 3.3.1 SP1.
The mass spectral data were searched against the Swiss-Prot databases of human and mouse proteins using the SEQUEST algorithm as part of the Bioworks software (Thermo Scientific, San Jose, CA) with a full tryptic cleavage requirement and a static cysteine alkylation modification.
The generated MS/MS spectra were searched against the human database using the Sequest search engine in the Proteome Discoverer (Version 1.4) software, with the false discovery rate (FDR) setting to 0.01.
For peptide identifications, the generated MS/MS spectra were evaluated using the SEQUEST analysis program and/or Mascot.
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