Sentence examples for using the sequest algorithm from inspiring English sources
MS2 spectral assignment was performed using the SEQUEST algorithm using Uniprot Human reference proteome (UP000005640, downloaded 10/05/2016) and a list of known contaminants (CRAPome.org).org
MS/MS spectra were searched using the Sequest algorithm with serine, threonine, and tyrosine phosphorylation and methionine oxidation as dynamic modifications.
MS/MS spectra were analyzed using the SEQUEST algorithm [15] in conjunction with publicly available predicted protein sequences from the appropriate genome sequence.
MS/MS were searched using the SEQUEST algorithm [37] against a sub-database of human, mouse and rat proteins derived from the NCBI non-redundant protein database.
MS/MS raw data were used to search against the Hordeum vulgare protein database at NCBI (http://www.ncbi.nlm.nih.gov) using the SEQUEST algorithm incorporated into the BioWorks software (Version 3.2, Thermo Finnigan, San Jose, CA, USA).
Tandem mass spectra were de-isotoped using in-house written software and data searched using the SEQUEST algorithm with no enzyme specificity for the variable modifications+15.99491 on methionine and+79.96633 on serine, threonine and tyrosine [53], [54].
The resulting tandem MS data were searched using the Sequest algorithms embedded in Proteome Discoverer 1.2 (Thermo) against a SwissProt/UniProtKB human protein sequence database (including 56,582 entries).
The MS/MS spectra were searched against the NCBI non-redundant protein sequence database using the SEQUEST computer algorithm [11] to produce a list of proteins identified in each sample.
The most intense peak was selected for MS/MS experiments, which were performed on a Thermofinnigan LCQ (ThermoQuest/Finnigan, San Jose, CA. USA) and the mass spectra were searched against databases using the Sequest search algorithm (http://www.thermoquest.com).
Mass spectra were searched against a protein database using the SEQUEST [ 25] algorithm in Bioworks 3.3 (Thermo Fisher Scientific).
For peptide identifications, the generated MS/MS spectra were evaluated using the SEQUEST analysis program and/or Mascot.
