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The 2nd screen genotyping was carried out using the Sequenom platform (iPlex and hME; http://www.sequenom.de/).sequenom.de/
Polymorphisms in the five genes of the adiponutrin gene family were selected and genotyped using the Sequenom platform in a childhood and adolescent obesity case-control study.
Genotyping of the SNPs in the FAAH (GeneID 2166) and MGLL (GeneID 11343) from dbSNP was performed using the Sequenom platform.
In total, 61 out of 85 selected SNPs were successfully genotyped using the Sequenom platform in a childhood and adolescent obesity case-control material (Table S1 and Figures S1, S2, S3, S4, S5).
Additionally, 38 SNPs from three genes (plasminogen [PLG], methionine synthase reductase [MTRR] and tissue factor pathway inhibitor [TFPI]) were successfully genotyped (genotyping efficiency >95%) in the Cenn samples, using the Sequenom platform.
The Australian MS trio families were genotyped using the SEQUENOM platform at The Australian Genome Research Facility We designed intronic primers flanking each of the exons for all the genes located in the critical interval, using Primer3 software.
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Studies by Thomas and colleagues [ 3], MacConaill and colleagues [ 36], and Dias-Santagata and colleagues [ 37] examined between 250 and 1,000 breast cancer and other tumor specimens for 120 to 400 mutations in 13 to 33 known oncogenes and tumor-suppressor genes by using the Sequenom platforms.
Genotyping on genomic DNA was performed either at deCODE Genetics using the Centaurus (Nanogen) platform or at the McGill University and Genome Quebec Innovation Centre using the Sequenom MassARRAY platform (San Diego, CA, USA).
The polymorphisms of AS3MT, GSTO, and PNP were performed using the Sequenom MassARRAY platform with iPLEX Gold chemistry.
The SNPs were tested by using the Sequenom genotyping platform (Sequenom®, San Diego, CA, USA), which uses MALDI-TOF mass spectroscopy and MassARRAY technology with an iPlex system.
Genotyping was performed using the Sequenom iPLEX platform.
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