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DCE-MRI was performed using the sequences described below.
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We used the sequences described by Green et al. [39] for measuring ALS3 RNA levels: ALS3 FOR: 5'-CCACTTCACAATCCCCATC-3', and ALS3 REV: 5'-CAGCAGTAGTAGTAACAGTAGTAGTTTCATC-3'.
Axial MR images were acquired through the centre of the gel using the 2 sequences described previously.
The 1R method was applied to each algorithm using the sequence sets described above (80 positives plus 185 negatives), with bin size set to the smallest possible value between 2 and 10 allowing separation into exactly two classes.
For the COG analysis, we constructed a local COG database [ 40] and ran RPSBLAST [ 37] using the sequence sets described above as queries with an e-value cutoff of 0.001.
To maintain our animal colony, founders were bred to wild-type mice and progeny scored by PCR genotyping for both mutations on the same chromosome in cis using the primer sequences described [18].
The intergenic segments were amplified using the primers' sequences described by Millemann et al. [ 18] and Versalovic et al. [ 19].
PCR amplification was performed in ChIP fragments using the oligonucleotide sequences described previously.
The β-glucosidase gene (bgl), consisting of 1437 bp, was amplified from the genome of B. licheniformis CGMCC 2876 by PCR using the sequence-specific primers described above.
The SIDER1β sequences were divergent enough to be detected in the screening using the consensus sequence described by Bringaud F et al. [ 37] as a query.
We implemented the comparative biology stream during fall semester 2006 (F06) and spring semester 2007 (S07) using the instructional sequence described above.
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