Sentence examples for using the sequence pair from inspiring English sources

Using the sequence pair representation, we derived and proved some necessary conditions for feasible buses, for which we allow 0-bend, one-bend, or two-bend.

To calculate fusion or fission results we used the sequence pairs identified in T. brucei as fusion-linked (see above) and searched for homologues against all the available sequences in the NCBI database of all organism family groups shown in the tree.

Positive clones were sequenced on an ABI sequencer using the sequencing primer pair M13 47 and M13 48 (see Table S2, Supplementary Material online).

To estimate the synonymous (Ks) and non-synonymous substitutions (Ka) rates between paralogous and orthologous sequences pairs, we first aligned the sequence pairs using tblastx; sequences producing significant alignments were extracted using their aligned coordinates and further analysed by translation and then amino acid sequence alignment was performed by Clustalw.

To estimate the Ks for common carp paralogs, we first aligned the sequence pairs using TBLASTX and then followed the same steps that were used in the Ks estimation pipeline in orthologous pairs.

Therefore we subsequently used LALIGN to re-analyse the sequence pairs showing SSEARCH matches, and identified a further 791 pairwise local alignments.

The chromosomal DNA of isolate 06-01-003MEF (CC19A, serotype 19A) was sequenced using the Solexa paired-end sequencing platform (Illumina, San Diego, CA).

The hypervirulent isolate's genome was sequenced using the Solexa paired-end sequencing platform and compared to that of a reference serotype 19A isolate, revealing the presence of a novel 20 kb region with sequence similarity to bacteriophage genes.

The Clostridium butyricum CWBI 1009 genome was sequenced by BaseClear (Leiden, The Netherlands) using the pair-end sequencing on the Illumina Hiseq 2000.

The entire ITS region (including the 5.8S gene) was amplified and sequenced using the primer pair P17F/26-82R and P16F/P25R (Popp and Oxelman [2001]).

Alongside this, direct plasmid sequencing using the primer pair R3: 5′-AAT TAGCCCGGCGCGGCG GAT-3′ and L3: 5′-GGC CGC GGC CGG CCG TCG −3′ wandcarried out to estimate the size and determine the map position of the insert.