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Both strands for each overlapping fragment were assembled using the sequence editing software Geneious v3.7 [ 84].
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The mtDNA sequences were edited using the sequence alignment editor BioEdit 7.0.5.3 (Hall 1999) and aligned using the program Clustal X (Larkin et al. 2007).
The final alignments were manually inspected and edited using the sequence alignment editor SE-Al version 1.d1 software [43].
Total COI sequences were organized using the multiple-sequence editing program BioEdit version 7.0.5.3 (Hall, 1999) and aligned visually.
The sequences were edited using the Sequencer program (version 3.1) (GeneCodes).
Consensus sequences were constructed by merging the alignment results using a sequence editing commercial software, Genetyx (Genetyx Inc ,Tokyo Japan).
The sequences obtained were trimmed using the Bio Edit Sequence alignment editor software (Ibis Biosciences, Carlsbad, CA, USA).
Alignments were manually refined using BioEdit sequence editing software [ 43].
Sequences were aligned using ClustalX [ 46] and manually refined using BioEdit sequence editing software [ 47].
We performed cycle sequencing and reaction clean-up following Werth and Sork [28] and edited sequences using the program Sequence Scanner, version 1.0 (Applied Biosystems, Foster City, CA, USA).
The program can be used for sequence editing, annotation of sequence features, handling of output from high throughput sequencers, or from BLAST searches, as well as for various population genetic analyses.
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