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Finally, all TaqMan PCR data were captured using the Sequence Detector Software (SDS version 1.9, Applied Biosystems, US).
The TaqMan readings were analyzed using the "Sequence Detector" v1.6.3 software (Applied Biosystems).
PCR was performed using the ABI PRISM 7900HT Sequence Detection System using the Sequence Detector Software version 2.2 (Applied Biosystems, Foster City, CA, USA).
Real-time PCR data were analyzed using the Sequence detector version 2.2.2 software supplied with the 7900 HT Fast-Real-Time Fast-Real-Time Fast-Real-Time PCR System Applied Biosystems
Real-time PCR amplification was performed using an Applied Biosystems 7900HT Real-Time PCR System and analyzed using the sequence detector software.
Data were analyzed using the Sequence Detector v1.9 software, and statistical analyses were performed using the R-statistical computing programming language (R Development Core Team 2006 R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
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All qRT-PCR reactions were performed using the following conditions: 50°C for 2 min, then 95°C for 10 min followed by 45 cycles of denaturation at 95°C for 15 s, annealing and extension at 60°C for 1 min. Amplification plots were generated as fluorescence of SYBR Green I (Rn) vs. PCR cycle number using the Sequence Detector Software v. 1.7 (Applied Biosystems).
Real-time PCR was performed using the 7900HT Sequence Detector and the Power SYBR Green PCR Master Mix (Applied Biosystems).
To quantify the expression of mouse TRP channel genes, we applied the TaqMan PCR system using the 5700 Sequence Detector (Applied Biosystems).
The DNA templates were assessed for HPV16 gene copy by real-time quantitative PCR using the ABI7700 sequence detector (Applied Biosystems, CA, USA).
For cohort III, RNA expression levels in human PBMCs were measured by using the ABI 7900HT Sequence detector (Sequence Detector Software 2.2.3) according to manufacturer's instructions.
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