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This, in turn, shows that using the sequence bootstrap to infer errors on divergence times [17], [18] is not comprehensive and therefore the CIs are too narrow.
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Using the sequence alignment, the bootstrapped neighbor joining trees (positions with gaps removed and corrected for multiple substitution) were constructed using clustalw2 (version 2.1) (Larkin et al., 2007).
The reliability of internal branches was assessed using the bootstrapping method (400 bootstrap replicates).
Node support of phylogenetic tree was assessed using the bootstrap method with 100 bootstrap replicates.
E[ p] is the expected value of p using the bootstrap technique for 100 000 bootstraps.
5 Ninety-nine perCIst CIs were calculated using the bootstrap percentile method with 2,000 bootstrap samples.
Strict consensus trees were constructed, and bootstrap values were calculated as measures of branch support [ 79] using the following bootstrap parameters: 100 bootstrap replicates, 5000 MAXTREES per replicate, random addition sequence, TBR, STEEPEST DESCENT, and MULTREES.
The Oncorhynchus clade in the amino acid tree was poorly resolved by Phyml and little inference could be gained, however O. kisutch was placed as an outgroup to the clade by the ML method when using the DNA sequences with high bootstrap support.
95% CIs are computed using the bias-corrected bootstrap method using 1000 bootstrap samples.
ML branch support was assessed via 100 nonparametric bootstrap pseudoreplicates, using the "rapid" bootstrap approach.
Bootstrap values were calculated using the rapid bootstrap method and CAT model with 100 replicates.
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