Sentence examples for using the selected primers from inspiring English sources

The cDNA amplifications were performed using the selected primers pairs mentioned above.

Real-time RT-PCR reactions were performed using the selected primers (Table 2) and a DNA Engine Opticon Continuous Fluorescence Detection System (see Methods).

The primers used to generate probes for Southern hybridization to genomic DNA are listed in Table 5. PPE-MPTR and mycP probes were generated using the selected primers to individually PCR amplify regions from the PPE-MPTR genes Rv1917c (PPE34) and Rv1753c (PPE24), as well as from the mycosin gene mycP5 (Rv1796).

The primer specificity was further evaluated by amplifying DNA samples from three waxy and three non-waxy sorghum varietals using the selected primer pairs described above.

The selected primers were used in a qPCR assay, and the calibration curves were linear from 5×× 101 to 5 × 105 copies mL−1, with a high correlation coefficient (R2 = 0.999).

In order to obtain reliable phylogenetic results, we carried out the alignment by using identical number of bp for each of the obtained sequences (465 bp for archaeal amoA and 640 bp for bacterial amoA), which were almost the full fragments amplified by the selected primer sets used in our study.

DNA was extracted from all of the products, and the amplification of waxy DNA was tested using the three selected primer pairs.

To test if the assay could distinguish between homozygous and hemizygous plants, eight samples were screened using the four selected primer pairs mentioned above.

> The RAPD analysis yielded a total of 232 loci for 130 individuals generated by using 20 selected primers, of which 78.9 % (183 fragments) were polymorphic between individuals [see Supporting Information— Table S4 ].

The use of selected primers caused the desired reduction in rRNA reads non-ribosomal reads improving from 11% with random hexamers to 45% with selected hexamers.

SSR markers were amplified using selected primer pairs (PP; described in the next section) on Biosystems 2720 thermal cyclers (Applied Biosystems, CA), using the PCR reaction conditions as described by Wang et al. (2011).