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All images were segmented using the same light threshold, mask smoothing and object size filters.
3D confocal images were segmented using the Otsu Thresholding algorithm [34] using the ITK software [35].
Automatic threshold-based segmentation will be refined based on these techniques, to find a balance between the threshold used and the segmented volume.
It imposes the constraint that segment separation be achieved using the same threshold for separating every group from its parent.
We use the score threshold to limit the number of segments that are retained in the search process.
18 19 Briefly, CMR data are segmented using the commercial software Mimics (Materialise, Leuven, Belgium), the thresholding being based on the pixel greyscale.
Another algorithm based on the theory of quasispecies was reported by Wan et al. [ 51], where using segment genotype thresholds allowed the identification of reassortment events.
We also surveyed birds in the plantation matrix and treated the patch area of the matrix as 0. We examined whether the detection rates of species changed suddenly across patch area (threshold) using segmented regression.
Each nucleus was segmented from the background using a global threshold and the segmented nuclei were stored in galleries in each case.
For the calculation of the morphometric parameters, the images were segmented using a uniform threshold as described in a previously published paper [ 6].
The image was segmented by thresholding and then analyzed using the analyze particle tool (size: 0-infinity, circularity: 0 1).
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