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The utility of the MeDIP-chip dataset may be limited because only a single control heart was used, but we have also used a second series of hearts and verified using the second methodology of bisulphite sequencing, that differential methylation exists in heart failure.
Due to this change in assay methodology, insulin levels from participants' samples (N = 258) assayed using the first methodology were corrected to levels in samples assayed using the second methodology (as per correction factor equation of the laboratory).
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Using the first methodology, nucleotides 10478 10507 were deleted from the 3' UTR (Δ30) of a wild type cDNA clone for DEN-4 to generate a vaccine candidate, rDEN4Δ30, which is safe, attenuated, and immunogenic in rhesus monkeys and humans [ 13].
As shown in Table 3, the annual DNI calculated for Ghardaia using the first model of the methodology is the most accurate value (2,409 kWh/m2) with a percentage error of −0.406 %, and the worst one is that obtained by the third model (2,083 kWh/m2) that implies a percentage error of −13.863 %.
For this mode, the model was solved using the first solution procedure described in "Methodology".
Using the third solution procedure described in "Methodology", the technical economic feasibility evaluation by Homer software was repeated for a wind power plant in Taze-Abad andanothernother plant in Sumar area.
The second methodology uses system reliability methods.
The second methodology used to test the Ambush Hypothesis is more direct and compares the actual frequency of OSCs in a genome with the frequency expected by chance based on the coding properties of that genome [ 4].
The second methodology uses a microbubble-based ultrasound contrast agent (UCA) and is based on the fact that the change in the acoustic properties of UCA depends on blood pressure (Forsberg et al. 2005; Dave et al. 2012).
In order to estimate the ANC calibrations needed in these countries, a review was undertaken using the methodology first developed by Gouws et al.[ 14] with an updated set of 48 population prevalence surveys in 24 countries.
According to the methodology used, the first step is to determine the available controllable load of the distribution utility/area under consideration.
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