Sentence examples for using the second generation from inspiring English sources

A set of 3 lentiviral vectors containing shRNA targeted to the DGKζ gene (Thermoscientific; Catalogue no. RHS4531-EG8525) were used to generate lentivirus using the second generation packaging plasmids pMD2.G and psPAX2 from Addgene.

Atomistic calculations were performed using the second generation forcefield COMPASS as well as the general purpose forcefields Dreiding and Universal.

The LNA NF can be further reduced by using the second generation enhanced SiGe C HBT BiCMOS process.

A series of dendronized diblock copolymers having rigid backbone and reactive surface were synthesized by ring-opening metathesis polymerization (ROMP) from dendronized norbornene derivatives using the second generation Grubb's catalyst.

Using the second generation Grubbs' catalyst RuCl2(C21H26N2)(CHPh PCy3 (Cy = cyclohexyl) and Hoveyda Grubbs' catalyst RuCl2(C21H26N2){CH-C6H4 O-iPr -2}, the reactions were performed with various C-allylated and O-allylated derivatives of trimethylhydroquinone-1-acetate as substrates.

Lentiviral packaging was achieved using the second generation packaging plasmid pCMV-dR8.2 dvpr and pCMV-VSV-G (Addgene Cat. No. 8455 and 8454).

Experimental results taken using the first generation fixed-angle arrangement will be presented along with the rationale for building the second.

We obtained a recovery of FNRBCs (74.0 ± 6.3%, p < 0.05, n = 5) using the third generation device, with a depletion of 46.5 ± 3.2% AARBCs from the cell mixture.

While developing a second generation of the affinity purification method [8], we showed that by using the first generation method we successfully purified RNA molecules as long as ∼341 nt.

Lentiviruses were produced using the third generation HIV-pseudotyped system [ 6].

The HCV antibody status was examined using the third generation of HCV enzyme immunoassay (EIA version 2.0; Abbott Laboratories, Abbott Park, IL).