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As the clearing of the liquid medium has been used as a criterion during the anti-infective screening, we also performed the MIC microdilution test using the screening medium.
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Since acetic acid, HMF and furfural were three major products during lignocellulose pretreatment and the most toxic inhibitors to microbial growth [ 3, 16], microbial growth behaviors on the screening medium could be used as an index of toxin tolerance.
The screening medium (unless otherwise specified) was used in all the optimization experiments mentioned above.
The screening medium without cell inoculation served as the control.
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The plant-based essential oils were evaluated for in vitro antifungal activity against Fusarium spp. using the Toxic Medium Assay (TMA) for preliminary screening.
For instance, Awale et al. reported that using the nutrition depleted medium screen platform, natural compound arctigenin could eliminate the tolerance of pancreatic cancer cells to nutrient starvation.
Dimethyl sulfoxide (DMSO, 2%) in the screen medium served as the negative control, and amphotericin B and ketoconazole in screen medium were used individually as positive antifungal controls.
MRSA agar was used as a screening medium for the determination of methicillin resistance and oxacillin resistance in Staphylococcus aureus.
Although SurePath is a widely used screening medium for sample collection, no previous study has addressed the use of CLART technology for HPV detection on this media type, using automated extraction and testing.
A defined synthetic medium with (g/L) d-glucose (or another carbon source) 20, NH4Cl 7.5, MgSO4·7H2O 0.5, NaCl 1.0, and KH2PO4 3.5 was used as the basal screening medium for the pellet formation.
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