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We used Perl scripts to perform BLAT alignments [ 21] using the Sanger trace files obtained during the sequencing.
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Using the Sanger strategy, the genome sequence of PA64s now comprises 382 Mb.
The E. coli DH10B strain was used as the cloning host, and six clones were selected to be sequenced using the Sanger method at MACROGEN (Seoul, South Korea).
PCR products of the bacterial DNA were sequenced using the Sanger method of sequencing with 3500 ABI genetic analyser, at Inqaba Biotechnical Industries, South Africa.
PCR products were sequenced using the Sanger method.
The p.Q485H mutation identified via WES was confirmed using the Sanger method.
Each sequence generated using the Sanger method had a perfect match to overlapping fragments.
Sequencing was done at the DOE Joint Genome Institute JGII) using the Sanger method.
> A short insert library was sequenced using the Sanger procedure.
These consisted of 305 true SNVs (variants seen in the Sanger traces) and 192 that could not be confirmed in the Sanger traces.
Gaps were initially verified using Sanger trace files available for isolates J0161, J2818 and F6854 and sequencing by synthesis technologies data files.
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