Sentence examples for using the sanger technology from inspiring English sources
After plating, we sequenced 10,063 randomly picked clones using the Sanger technology (ABI 3730) and the M13-FP primer.
After plating, we sequenced 83,152 randomly picked clones using the Sanger technology (ABI 3730) and the SP6 primer.
Most of these sequences were non-annotated ESTs sequenced using the Sanger technology from a single study [ 33].
Polybayes.pl is a software designed to identify single nucleotide polymorphism (SNP) within assemblies of sequences produced using the Sanger technology that takes quality values into account [ 42].
Expressed Sequenced Tag (EST) sequencing using the Sanger technology has been extensively used to provide a first catalog of a species' gene inventory, and it is still the most used method to obtain transcriptome data for microarray construction.
The availability of SNPs collections for melon has increased in the past few years with the sequences produced by several national and international projects using the Sanger technology [ 20- 22].
As our objective was to identify the major venom proteins, and since no reference genome was available, we decided to use the Sanger technology to produce long, high quality sequences (Additional file 1: Figure S1).
Given the relatively small size and absence of repeats, sequencing and assembling the mitochondrial human genome was not as challenging and difficult as sequencing the human nuclear genome using the Sanger Sequencing technology.
To further assess the specificity of AsmVar in structural variation discovery, we randomly select one Danish trio from the Genome Denmark consortium and validate 272 novel structural variants with a range of different sizes (≥50 bp) and formation mechanisms using the Sanger sequencing technology [ 14].
The genome sequence of B. pinnipedialis B2/94 was determined (30× coverage) by shotgun sequencing with the GS-FLX technology and the remaining gaps filled using the standard Sanger technology.
The chromosome A3 in B. rapa has been sequenced using the traditional Sanger technology.
