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Using the Sanger strategy, the genome sequence of PA64s now comprises 382 Mb.
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The entire coding sequence of MYOC was amplified and sequenced using the Sanger method.
The PCR products were also sequenced using the Sanger method for validation.
PCR products were sequenced using the Sanger method.
The p.Q485H mutation identified via WES was confirmed using the Sanger method.
Each sequence generated using the Sanger method had a perfect match to overlapping fragments.
Sequencing was done at the DOE Joint Genome Institute JGII) using the Sanger method.
> A short insert library was sequenced using the Sanger procedure.
Those portions with missing data were resequenced using the nested PCR strategy described earlier and Sanger sequencing, which also allowed verifying the concordance of the two sequencing procedures.
Sequencing was completed using the standard Sanger method as described by Sanger et al. [ 49].
The cDNAs were sequenced using the conventional Sanger sequencing.
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