Sentence examples for using the sanger platform from inspiring English sources

We compared our GA2-generated assemblies to EST collections generated previously using the Sanger platform [ 22- 25].

Using ClustalW alignment and PCR confirmation, we found a 282 bp deletion in the region of ndhA intron using the Sanger platform and a 152 bp insertion in the inverted repeats using the Illumina MiSeq platform (Additional file 2: Figure S1 and Additional file 3: Figure S2).

Genomic sequences of the family members of the patients were also confirmed using the Sanger sequencing platform after PCR amplification of the corresponding exons to confirm the results.

Using the Sanger strategy, the genome sequence of PA64s now comprises 382 Mb.

PCR products were sequenced using the Sanger method.

Using the Sanger data, we found that the sequence identities among the three sequences were extremely similar, but the lengths of the three contigs from Sanger, Illumina MiSeq and PacBio RS II sequencing platforms were different.

Sequencing was done at the DOE Joint Genome Institute JGII) using the Sanger method.

> A short insert library was sequenced using the Sanger procedure.

We used the Sanger, 454 Titanium, and Illumina GA or Illumina HiSeq2000 platforms to generate transcriptome sequences for lodgepole pine and jack pine (Additional file 1: Table S1).

Recently, similar efforts were made in plants by using the Solexa/Illumina platform for re-sequencing of Arabidopsis thaliana [ 14] and by a mixed Sanger and 454/Roche sequencing strategy for grapevine (Vitis vinifera) [ 15].

The draft genomes were analysed for accuracy and coverage using the BIGSdb platform [ 17] which enabled comparison with 24 antigen and MLST typing loci previously characterised with Sanger sequencing and four finished reference genomes, cross-validating these technologies.