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Using the Sanger miRNA database, we examined the chromosomal distribution of miRNAs that were altered in 10-87 LP and 10-87 HP cells.
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Using the Sanger strategy, the genome sequence of PA64s now comprises 382 Mb.
PCR products were sequenced using the Sanger method.
Using the Sanger miRBase Target v5 and TargetScan v4.2, we found 1578 and 1899 predicted targets, respectively, for the group of highly expressed miRNAs from plasma microvesicles (Table S2).
Sequencing was done at the DOE Joint Genome Institute JGII) using the Sanger method.
> A short insert library was sequenced using the Sanger procedure.
MiRNA precursor sequences and mature sequences where taken from the Sanger miRNA Registry (version 10.0).
Human miRNA and its corresponding pri-miRNA sequences were obtained from the Sanger miRNA Registry (http://www.sanger.ac.uk/software/Rfam/mirna).sanger.ac.uk/software/Rfam/mirna
To date, 10883 miRNA sequences have been published on the Sanger miRNA Registry (http://www.sanger.ac.uk/software/Rfam/mirna, miRbase Release 14.0).
Seeds (nt 1 8 and 2 9) of all 470 human miRNAs listed in the Sanger miRNA Registry (http://microrna.sanger.ac.uk, version 10.0), were selected.
Each region further contained a miRNA probe region detecting miRNA transcripts listed in the Sanger miRNA database (http://www.sanger.ac.uk/software/Rfam/mirna/). Multiple and TM-normalized control probes were included on each chip.
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